Double-stranded RNA induces disproportionate expression of thymic stromal lymphopoietin versus interferon-β in bronchial epithelial cells from donors with asthma
Identifieur interne : 001644 ( Main/Exploration ); précédent : 001643; suivant : 001645Double-stranded RNA induces disproportionate expression of thymic stromal lymphopoietin versus interferon-β in bronchial epithelial cells from donors with asthma
Auteurs : Lena Uller [Royaume-Uni, Suède] ; Marina Leino [Royaume-Uni] ; Nicole Bedke [Royaume-Uni] ; David Sammut [Royaume-Uni] ; Ben Green [Royaume-Uni] ; Laurie Lau [Royaume-Uni] ; Peter H. Howarth [Royaume-Uni] ; Stephen T. Holgate [Royaume-Uni] ; Donna E. Davies [Royaume-Uni]Source :
- Thorax [ 0040-6376 ] ; 2010-07.
English descriptors
- Teeft :
- Airway, Airway epithelial cells, Allergy clin immunol, Antiviral response, Asthma, Asthma group, Asthma groups, Asthmatic, Bars show, Becs, Bronchial epithelial cells, Chloroquine, Clin, Cytokine, Dsrna, Epithelial, Epithelial cells, Epithelium, Gene expression, Gure, Healthy controls, Healthy group, Healthy subjects, Ifnb, Ifnb expression, Ifnb mrna, Immunol, Inhibitor, Interferon, Interquartile range, Lymphopoietin, Median, Median values, Mrna, Mrna expression, Ndings, Online, Online supplement, Open circles, Plots show, Present study, Protein expression, Protein synthesis, Receptor, Rhinovirus, Severe asthma, Stromal, Thorax, Thymic, Thymic stromal lymphopoietin, Time points, Tlr3, Tslp, Tslp expression, Tslp gene expression, Tslp mrna, Tslp mrna expression, Tslp production, Viral, Viral infection, Wilcoxon rank.
Abstract
Background Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine that strongly activates dendritic cells and can initiate allergic inflammation. Since exposure to rhinovirus or double-stranded (ds) RNA (a surrogate of viral infection) induces TSLP expression in bronchial epithelial cells (BECs), this cytokine may link innate antiviral responses and the type 2 adaptive immune response. Objective As BECs from donors with asthma have a deficient interferon (IFN) response to rhinovirus infection, a study was undertaken to test the hypothesis that their antiviral response shows a bias towards TSLP production. Methods Primary BECs were grown from subjects with asthma and healthy volunteers. After exposure to dsRNA, interleukin (IL)-8, IFNβ and TSLP mRNA and protein expression were measured by RT-qPCR and ELISA, respectively. Results dsRNA dose-dependently increased IL-8 expression in BECs with no significant difference between the groups. However, BECs from subjects with asthma expressed less IFNβ and more TSLP mRNA and protein in response to dsRNA than BECs from those without asthma (median (IQR) 57 (38–82) pg/ml vs 106 (57–214) pg/ml for IFNβ (p<0.05) and 114 (86–143) pg/ml vs 65 (32–119) pg/ml for TSLP (p<0.05) in response to 10 μg/ml dsRNA for 24 h). Induction of TSLP mRNA by dsRNA was blocked by Toll-like receptor 3 or protein kinase inhibitors or by preventing de novo protein synthesis, but not by neutralisation of type I IFN receptors. Conclusion BECs from subjects with asthma are biased towards higher TSLP and lower IFNβ production in response to dsRNA, suggesting that viral infection in asthma may lead to an altered mediator profile that biases towards a Th2 immune response.
Url:
DOI: 10.1136/thx.2009.125930
Affiliations:
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Howarth</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Brooke Laboratories, Division of Infection, Inflammation and Repair, School of Medicine, University of Southampton, Southampton</wicri:regionArea><wicri:noRegion>Southampton</wicri:noRegion></affiliation></author><author><name sortKey="Holgate, Stephen T" sort="Holgate, Stephen T" uniqKey="Holgate S" first="Stephen T" last="Holgate">Stephen T. Holgate</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Brooke Laboratories, Division of Infection, Inflammation and Repair, School of Medicine, University of Southampton, Southampton</wicri:regionArea><wicri:noRegion>Southampton</wicri:noRegion></affiliation></author><author><name sortKey="Davies, Donna E" sort="Davies, Donna E" uniqKey="Davies D" first="Donna E" last="Davies">Donna E. Davies</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Brooke Laboratories, Division of Infection, Inflammation and Repair, School of Medicine, University of Southampton, Southampton</wicri:regionArea><wicri:noRegion>Southampton</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Thorax</title><title level="j" type="abbrev">Thorax</title><idno type="ISSN">0040-6376</idno><idno type="eISSN">1468-3296</idno><imprint><publisher>BMJ Publishing Group Ltd and British Thoracic Society</publisher><date type="published" when="2010-07">2010-07</date><biblScope unit="volume">65</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="626">626</biblScope></imprint><idno type="ISSN">0040-6376</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-6376</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Airway</term><term>Airway epithelial cells</term><term>Allergy clin immunol</term><term>Antiviral response</term><term>Asthma</term><term>Asthma group</term><term>Asthma groups</term><term>Asthmatic</term><term>Bars show</term><term>Becs</term><term>Bronchial epithelial cells</term><term>Chloroquine</term><term>Clin</term><term>Cytokine</term><term>Dsrna</term><term>Epithelial</term><term>Epithelial cells</term><term>Epithelium</term><term>Gene expression</term><term>Gure</term><term>Healthy controls</term><term>Healthy group</term><term>Healthy subjects</term><term>Ifnb</term><term>Ifnb expression</term><term>Ifnb mrna</term><term>Immunol</term><term>Inhibitor</term><term>Interferon</term><term>Interquartile range</term><term>Lymphopoietin</term><term>Median</term><term>Median values</term><term>Mrna</term><term>Mrna expression</term><term>Ndings</term><term>Online</term><term>Online supplement</term><term>Open circles</term><term>Plots show</term><term>Present study</term><term>Protein expression</term><term>Protein synthesis</term><term>Receptor</term><term>Rhinovirus</term><term>Severe asthma</term><term>Stromal</term><term>Thorax</term><term>Thymic</term><term>Thymic stromal lymphopoietin</term><term>Time points</term><term>Tlr3</term><term>Tslp</term><term>Tslp expression</term><term>Tslp gene expression</term><term>Tslp mrna</term><term>Tslp mrna expression</term><term>Tslp production</term><term>Viral</term><term>Viral infection</term><term>Wilcoxon rank</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Background Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine that strongly activates dendritic cells and can initiate allergic inflammation. Since exposure to rhinovirus or double-stranded (ds) RNA (a surrogate of viral infection) induces TSLP expression in bronchial epithelial cells (BECs), this cytokine may link innate antiviral responses and the type 2 adaptive immune response. Objective As BECs from donors with asthma have a deficient interferon (IFN) response to rhinovirus infection, a study was undertaken to test the hypothesis that their antiviral response shows a bias towards TSLP production. Methods Primary BECs were grown from subjects with asthma and healthy volunteers. After exposure to dsRNA, interleukin (IL)-8, IFNβ and TSLP mRNA and protein expression were measured by RT-qPCR and ELISA, respectively. Results dsRNA dose-dependently increased IL-8 expression in BECs with no significant difference between the groups. However, BECs from subjects with asthma expressed less IFNβ and more TSLP mRNA and protein in response to dsRNA than BECs from those without asthma (median (IQR) 57 (38–82) pg/ml vs 106 (57–214) pg/ml for IFNβ (p<0.05) and 114 (86–143) pg/ml vs 65 (32–119) pg/ml for TSLP (p<0.05) in response to 10 μg/ml dsRNA for 24 h). Induction of TSLP mRNA by dsRNA was blocked by Toll-like receptor 3 or protein kinase inhibitors or by preventing de novo protein synthesis, but not by neutralisation of type I IFN receptors. Conclusion BECs from subjects with asthma are biased towards higher TSLP and lower IFNβ production in response to dsRNA, suggesting that viral infection in asthma may lead to an altered mediator profile that biases towards a Th2 immune response.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>Suède</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Uller, Lena" sort="Uller, Lena" uniqKey="Uller L" first="Lena" last="Uller">Lena Uller</name></noRegion><name sortKey="Bedke, Nicole" sort="Bedke, Nicole" uniqKey="Bedke N" first="Nicole" last="Bedke">Nicole Bedke</name><name sortKey="Davies, Donna E" sort="Davies, Donna E" uniqKey="Davies D" first="Donna E" last="Davies">Donna E. Davies</name><name sortKey="Green, Ben" sort="Green, Ben" uniqKey="Green B" first="Ben" last="Green">Ben Green</name><name sortKey="Holgate, Stephen T" sort="Holgate, Stephen T" uniqKey="Holgate S" first="Stephen T" last="Holgate">Stephen T. Holgate</name><name sortKey="Howarth, Peter H" sort="Howarth, Peter H" uniqKey="Howarth P" first="Peter H" last="Howarth">Peter H. Howarth</name><name sortKey="Lau, Laurie" sort="Lau, Laurie" uniqKey="Lau L" first="Laurie" last="Lau">Laurie Lau</name><name sortKey="Leino, Marina" sort="Leino, Marina" uniqKey="Leino M" first="Marina" last="Leino">Marina Leino</name><name sortKey="Sammut, David" sort="Sammut, David" uniqKey="Sammut D" first="David" last="Sammut">David Sammut</name></country><country name="Suède"><noRegion><name sortKey="Uller, Lena" sort="Uller, Lena" uniqKey="Uller L" first="Lena" last="Uller">Lena Uller</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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